Before writing a program, you must understand the variables you control. Every HPLC program consists of five fundamental "time-based" tables.
| Issue | Potential Cause | Program-Related Factor | | :--- | :--- | :--- | | Aspirin tailing | Degradation or silanol interaction | Isocratic 60% methanol may not maintain optimal pH (needs ~pH 3.0) | | Low plate count (Aspirin) | High solvent strength | 60% methanol is too strong, reducing retention and efficiency | hplc program
Setting the speed at which the mobile phase travels through the column. Before writing a program, you must understand the
Run completed. Awaiting new sequence.